The 2nd edition of the book is revised, updated and efforts are made to enhance usefulness of the book for various courses. New subject matter is added to each chapter. Further this freshly updated 2nd edition contains five new chapters. They are: Biochemistry of apoptosis Biochemistry of cell cycle Biochemistry of Blood Organ function tests Biochemical Technology Apart from updating each chapter, new unsolved problems are added and in references books, reviews, research articles are included. Thus, the 2nd edition of the book contains 34 chapters, 536 references, 191 essay-type question.